Journal: Cell
Article Title: The gut microbiota mediates the anti-seizure effects of the ketogenic diet
doi: 10.1016/j.cell.2018.04.027
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Detailed methods are provided and include the following: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Akkermansia muciniphila ATCC ATCC BAA845 Parabacteroides merdae ATCC ATCC 43184 Parabacteroides distasonis ATCC ATCC 8503 Bifidobacterium longum ATCC ATCC 15707 Chemicals, Peptides, and Recombinant Proteins GGsTop, 3-[[(3-amino-3-carboxypropyl) methoxyphosphinyl]oxy]benzeneacetic acid Tocris Bioscience 4452; CAS: 926281-37-0 Mucin from porcine stomach, type III Sigma-Aldrich M1778; CAS: 84082-64-4 L-gamma-glutamyl-3-carboxy-4-nitroanilide Gold Bio G-380-1; CAS: 63699-78-5 4 kDa FITC-dextran Sigma-Aldrich 46944; CAS: 60842-46-8 Critical Commercial Assays Glucose Colorimetric Assay Kit Cayman Chemical Cat# 10009582 Beta-hydroxybutyrate Colorimetric Assay Kit Cayman Chemical Cat# 700190 MoBio PowerSoil Kit Mo Bio Cat# 12888-100 Deposited Data 16S rDNA sequences This paper https://qiita.ucsd.edu , 11566 Experimental Models: Organisms/Strains Mouse: Swiss Webster, germ-free Taconic Taconic: SW GF Mouse: Swiss Webster, specific pathogen-free Taconic Taconic: SW MPF Mouse: Kcna1−/−: C3HeB.129S7-Kcna1 tm1|em /J Dr. Bruce Tempel JAX: 003532 Oligonucleotides MUC1437: 6FAM-CCTTGCGGTTGGCTTA Derrien, et al., 2008 Sigma Aldrich: custom oligo BAC303: Texas Red-CCAATGTGGGGGACCTT Manz, et al., 1996 Sigma Aldrich: custom oligo Software and Algorithms Ethovision XT 11 Noldus Cat # EX11-MAMBP-E QIIME1.8.0 Caporaso et al., 2010 http://qiime.org/ Greengenes Lawrence Berkeley National Labs http://greengenes.lbl.gov/cgi-bin/nphindex.cgi PICRUSt Langille et al., 2013 http://picrust.github.io/picrust/ Ponemah V5.1 Data Sciences International https://www.datasci.com/products/software/ponemah Neuroscore Data Sciences International https://www.datasci.com/products/software/neuroscore TraceFinder 3.3 ThermoFisher Scientific OPTON-30491 ImageJ National Institutes of Health https://imagej.nih.gov/ij/ Other Ketogenic Diet Mouse Chow Envigo TD.07797.PWD Control Diet Mouse Chow Envigo TD.150300 Standard Diet Mouse Chow LabDiet # 5010 ECT Unit Ugo Basile Model # 57800 EEG transmitters Data Sciences International PhysioTel ETA-F10 Open in a separate window KEY RESOURCES TABLE CONTACT FOR REAGENT AND RESOURCE SHARING Further information and requests for resources and reagents should be directed to and will be fulfilled by the Lead Contact, Elaine Hsiao ( ude.alcu@oaishe ) EXPERIMENTAL MODELS AND SUBJECT DETAILS Mice 3–4 week old SPF wildtype Swiss Webster mice (Taconic Farms), GF wildtype Swiss Webster mice (Taconic Farms) and SPF C3HeB/FeJ KCNA1 KO mice (Jackson Laboratories) were bred in UCLA’s Center for Health Sciences Barrier Facility.
Techniques: Virus, Recombinant, Colorimetric Assay, Software, Control
